Processes for production of human epidermal growth factor by genetic engineering.

摘要

By culturing E. coli bearing a DNA sequence comprising a tryptophan regulatory gene, a gene coding for the N-terminal of trpE or trpL polypeptide and a specific synthetic gene coding for human epidermal growth factor, hEGF can be obtained as fused protein efficiently. hEGF can also be obtained as matured protein efficiently by culturing E. coli bearing a DNA sequence comprising a tryptophan regulatory gene, SD sequence of tryptophan L or E, ATG and a specific synthetic gene coding for human epidermal growth factor immediately downstream said ATG. Thus obtained hEGF is applicable to treatment of gastric ulcer or wounds.