Preparations containing hepatocyte growth factor and hyaluronic acid and methods of making and using same

摘要

Preparations containing hepatocyte growth factor (HGF) and hyaluronic acid (HA) and methods of making and using same. The HGF and HA preparations can be prepared together in solution as an injectable fluid without gelatinization, or impregnated within a porous hydrophilic matrix material with, or without, cross-linking of the HA with the matrix material. The preparations can be used as a dermal filler or to generate and promote healing of cartilage, vertebral discs, connective tissues such as tendons and ligaments and bone in vivo.

主权项

1. A method of regenerating damaged or defective tissue comprising:
making a preparation by cross-linking hyaluronic acid (HA) in situ within a porous matrix, with limited or no chemical cross-linking of the hyaluronic acid with a material forming the porous matrix, by soaking the porous matrix with the hyaluronic acid, with the addition of cross-linking heavy chains of inter-alpha inhibitor and Tumor Necrosis Factor Stimulating Gene-6, wherein hepatocyte growth factor (HGF) is added to the preparation during the HA cross-linking stage or the HGF is added after the HA cross-linking stage by soaking the preparation in a solution containing HGF, and placing the preparation containing HGF into a synovial joint of the mammal.