Method for production of human tissue type plasminogen activator.
摘要
<p>Human tissue type plasminogen activator (tPA) which is effective as a therapeutic agent is obtained at a high concentration by increasing the osmotic pressure of the cell culture medium or tPA producing medium using bicarbonate ion up to at least 350 milliosmole/liter optionally also supplementing the medium with an antiplasmin agent. Single-chain tPA is produced at a high concentration accordingly.</p>
