| 摘要 |
<p>A method for amplifying specific target nu leic acid sequences in a sample, the method comprising (a) contacting under hybridization conditional (i) a first primer nucleic acid and a second primer nucleic acids, one of said first or second primer nucleic acids being immobilized or immobilizable, the other is either immobilized or immobilizable or labeled, with (ii) a test sample containing target nucleic acid sequences, (b) contacting the resultant product of step (a) with an extender enzyme and at least one nucleotide residue for extension under conditions to elongate the resultant hybridized primer nucleic acids, and (c) denaturing the product of step (b), to produce immobilized target nucleic acid sequences, and (d) repeating at least once a cycle of steps (a), (b) and (c) with the product of step (c) of the previous cycle being used in place of (a)(ii). The above described method can be used to detect specific target nucleic acid sequences by using a test sample suspected of containing target nucleic acid sequences and determining if an amplified sequence is present. o</p> |
