METHODS OF EXTRACTING NUCLEIC ACIDS AND PCR AMPLIFICATION WITHOUT USING A PROTEOLYTIC ENZYME

摘要

METHODS OF EXTRACTING NUCLEIC ACIDS AND PCR AMPLIFICATION WITHOUT USING A PROTEOLYTIC ENZYME This invention provides a rapid and highly effective method for extracting nucleic acids from cells or virions without the use of proteolytic enzymes. Extraction is accomplished within a few minutes using a lysing composition comprising a buffer, a source of a DNA polymerase cofactor, a stabilizer and at least one nonionic surfactant which will release nucleic acids from cytoplasmic and nuclear membranes of cells or virions. The resulting mixture is heated to boiling for up to fifteen minutes, and the nucleic acids are recovered for amplification using polymerase chain reaction. No proteolytic enzyme is used in the extraction process.