VERFAHREN UND REAGENZ ZUR SPEZIFISCHEN BESTIMMUNG DES CHOLESTERINS DER HDL-FRAKTION.

摘要

Specific determination of HDL-cholesterol in presence of the LDL fraction of serum lipoproteins comprises treating with cholesterol esterase (CE) to release cholesterol which is oxidised with cholesterol oxidase (CO) and O2 to form H2O2, then kinetic measurement of H2O2 formation or of O2 consumption. The new feature is that measurement is carried out at 2-15 min after start of oxidase reaction at 20-40 deg C for a predetermined time interval. During measurement concns maintained in the reaction soln are: CE 0.05-30 u/ml; Co 0.1-50 U/ml; bile acid surfactant 1-20 mM and nonionic surfactant 0.1-10 g/l, while pH is 5-9. Also new is a reagent which provides the specified concns. of CO, CE and surfactants, plus pH 5-9 buffer and a system for photometric measurement of H2O2.