METHOD FOR PURIFYING FACTOR VIII AND PREPARATIONS OBTAINED

摘要

. Method for purifying factor VIII and preparations obtained. In the method for purifying factor VIII from cryoprecipitate, which is dissolved and then treated with alumina gel in the customary manner, - the extract is diluted to a protein concentration not exceeding approximately 5 g/l, in particular 4 g/l, and subjected to viral inactivation with solvent/ detergent, - the inactivated extract containing the solvent/ detergent is then subjected to chromatography on a weak anion exchange column which is hydrophilic in nature, - the factor VIII then being eluted with a dissociating buffer. Preparation obtained, having an activity of the order of at least 100 IU VIII:C/mg of protein.