| 摘要 |
The present invention provides methods and kits for determining the presence or amount of a substance by detection of a colloidal dye associated with agglutinated particles. The methods and kits are disclosed in direct and indirect (e.g., competitive) formats. In one aspect, the present invention provides methods and kits utilizing a single colloidal dye. In another aspect, methods and kits are provided which include two colloidal dyes, wherein one colloidal dye functions as a background-enhancing dye. A related aspect of the present invention provides methods and kits that include a colloidal dye and a non-water-soluble dye (which functions as a background-enhanching dye) attached to a microporous pad which permits differential migration of non-agglutinated and agglutinated latex particles. In yet another aspect, the present invention provides methods and kits utilizing a colloidal dye and a water-soluble dye, which functions as a background-enhancing dye. |
