| 摘要 |
A method to determine the presence or amount of peroxidase or hydrogen peroxide employing a sulfonephthalein dye or a phthalein dye having a major absorbance peak in the visible region, preferably at a wavelength from between about 500 nanometers to about 700 nanometers, and which absorbance peak is subject to a degree of oxidative extinguishment without strong alkali treatment, is disclosed. The method is performed by forming a reaction system comprising such sulfonephthalein dye or phthalein dye, and a test sample containing an unknown amount of (i) peroxide, (ii) a peroxidase, (iii) a compound which is capable of being oxidized to generate hydrogen peroxide, or (iv) a catalyst which participates in such oxidation, wherein the hydrogen peroxide causes peroxidase-catalyzed extinguishment of the absorbance peak of such dye whereby the unknown amount under determination can be determined by monitoring such extinguishment. |
