主权项 |
1. A method of reducing an α,β unsaturated compound, the method comprising:
(a) providing: (i) an α,β unsaturated compound selected from the group consisting of an α,β unsaturated ketone, an α,β unsaturated aldehyde, an α,β unsaturated nitrile, and an α,β unsaturated ester; and (ii) an enone reductase polypeptide comprising an amino acid sequence that is at least 80% identical to a SEQ ID NO:8, wherein the residue corresponding to X124 is an aromatic, non-polar or a constrained residue, particularly a non-polar or a constrained residue; and (b) contacting the α,β unsaturated compound with the enone reductase polypeptide under reaction conditions suitable for conversion of the α,β unsaturated compound to the corresponding saturated product compound selected from the group consisting of a saturated ketone, a saturated aldehyde, a saturated nitrile, and a saturated ester. |