| 摘要 |
A method and reagent for determining fibrinogen from undiluted plasma samples. The use of undiluted plasma as sample is made possible by the use of a specific peptide inhibitor of fibrin aggregation in a concentration which permits aggregation of fibrin but with reduced speed, so that coagulation time can be measured easily. The range of fibrinogen concentrations which can be measured is increased as compared to that of known methods, and the measurement can be fully automated without prediluting the sample. A biological fluid, e.g., undiluted plasma, is incubated with a reagent containing at least one inhibitor of fibrin aggregation in an amount effective to increase coagulation time to allow measurement of the fibrin concentration, e.g., a coagulation time of about 50 to 150 seconds at a fibrin concentration of 1 gram/liter, and thrombin, or a protease of analogous activity in an amount, which immediately converts all the fibrinogen into soluble fibrin, e.g., in an excess of at least 20 U per ml of plasma. The coagulation time is then determined.
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