Reagent for Determining alpha -amylase activity and method for determining alpha -amylase activity

摘要

A reagent for determining a-amylase activity, comprising a maltooligosaccharide derivative of the following formula <IMAGE> (I) (wherein either one of R1 and R2 is beta -galactopyranosil and the other is hydrogen, R3 is a group bonded to the reducing terminal glucose via a bond cleavable by alpha -amylase, which becomes a measurable substance upon cleavage of the bond, and n is an integer of 0-2), which does not comprise adjuvant enzymes; and a method for determining alpha -amylase activity which comprises use of the reagent. The reagent of the present invention does not require use of any adjuvant enzyme and is stable since the substrate is not exposed to the decomposition by an adjuvant enzyme. The substrate used in the present invention has high affinity for alpha -amylase. Thus, the reagent and the determination method of the present invention make it possible to determine alpha -amylase activity with high sensitivity.