| 摘要 |
The present invention relates to an assay procedure and/or device for directly measuring VLDL, LDL, HDL as well as apolipoproteins cholesterol in blood plasma and body fluids. The invention provides a method for direct quantitative determination of VLDL, LDL, HDL as well as apolipoproteins cholesterol in a sample of body fluid in which an antibody against the lipoprotein or apolipoprotein is partitioned in one of two phases of an aqueous two-phase system with or without the help of a partitioning enhancer by conjugation. A sample of body fluid, such as blood plasma, is applied to the aqueous two-phase system. After mixing and incubation for a short period of time, the phases are separated by gravity or centrifugation within several minutes. The phase containing the antibody and the targeted lipoprotein or apolipoprotein is removed. The targeted lipoprotein cholesterol is then directly determined by using an enzymatic procedure. |
