发明名称 |
CHEMILUMINESCENT DETECTION OF HYDROLYTIC ENZYMES USING AN ACRIDAN |
摘要 |
A chemiluminescent assay method, compositions and kits are described which use a protected phenolic enhancer compound which is deprotected by a hydrolytic enzyme and then enhances a chemiluminescent reaction. The reaction involves an acridan compound, preferably a derivative of an N-alkyl-acridan-9-carboxylic acid, which is activated to produce light by a peroxide compound and a peroxidase enzyme in the presence of the deprotected enhancer. The result is enhanced generation of light from the reaction. The hydrolytic enzyme is present alone or linked to a member of a specific binding pair in an immunoassay, DNA probe assay or other assay where the hydrolytic enzyme is bound to a reporter molecule.
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申请公布号 |
WO9607911(A1) |
申请公布日期 |
1996.03.14 |
申请号 |
WO1995US10952 |
申请日期 |
1995.08.30 |
申请人 |
LUMIGEN, INC. |
发明人 |
AKHAVAN-TAFTI, HASHEM;ARGHAVANI, ZAHRA;DESILVA, RENUKA |
分类号 |
G01N33/535;C07D219/02;C07D219/04;C07D219/06;C09B15/00;C09K11/07;C12Q1/28;C12Q1/34;G01N21/78;G01N33/543;G01N33/58;(IPC1-7):G01N33/535 |
主分类号 |
G01N33/535 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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