| 摘要 |
A method of separating bound labeled indicator from free labeled indicator in a layer of a test element for immunoassay. The method comprises: a) depositing sample containing a target immunoanalyte capable of binding to the labeled indicator or to an immobilized antibody in competition with the labeled indicator, onto an exterior surface of a test element in the presence of the labeled indicator and b) adding an amount of wash liquid to the exterior surface to form a pool of the liquid having a meniscus on the surface, the liquid penetrating the surface over an area bounded by a closed intersect edge formed between the pool meniscus and the surface, so that penetrating liquid can push free labeled indicator away from bound labeled indicator in a volume of the layer below the bounded area. The method is improved in that the step b) comprises i) depositing a first amount of wash liquid at a first location on the surface in a pool, (ii) allowing at least a portion of the pool to spread out through a first portion of the test element to wash out free labeled indicator, and then (iii) depositing a second amount of wash liquid at a second location on the surface different from the first location to spread out through a second portion of the test element different from the first portion, so that the intersect edge is displaced over the surface over time and the liquid flowing into the test element at the intersect edge sweeps through more of the target read area than is the case when washing from only the first location, thus reducing background signal due to non-separated free labeled indicator remaining in the volume.
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