Device and method for microscopy using light with differing physical properties

摘要

The invention relates to a device for microscopy, with at least one light source for providing illumination light, with a detection unit for detecting light radiated back from a sample, with a microscopy optical unit for guiding illumination light onto the sample and for guiding light radiated back from the sample in the direction of the detection unit and with, arranged in an illumination beam path, an excitation mask (40) for providing structured illumination. The device is characterized in that the excitation mask is a spatially structured filter, which is transparent to light with a first physical property and which impresses spatial structure onto light with a second physical property that is different from the first physical property. The invention moreover relates to a method for microscopy.

主权项

1. A device for microscopy, comprising:
at least one light source to provide illuminating light, the illuminating light including light with a first physical property and light with a second physical property different from the first physical property, wherein the first physical property is a first wavelength or a first wavelength range of the light and the second physical property is a second wavelength of the light differing from the first wavelength or a second wavelength range of the light differing from the first wavelength range, a detection unit to detect light radiated back from a specimen, a microscope optical system to guide illuminating light onto the specimen and to guide light radiated back from the specimen in the direction of the detection unit and an excitation mask arranged in an illumination optical path to provide a structured illumination, wherein the excitation mask is a spatially structured filter, which is transparent to light with the first physical property, and which imprints a spatial structure on light with the second physical property, and wherein illuminating light with the first physical property is transmitted substantially unchanged by the excitation mask.