METHOD OF PREPARING TULIP MICROBULBS FROM ISOLATED EMBRYOS UNDER IN VITRO CONDITIONS

摘要

FIELD: agriculture biotechnology. SUBSTANCE: isolated tulip embryos are cultured at first on agarized van-Hoff medium and then on modified Murasige-Skoog medium containing additionally, mg/l: nickel nitrate 2.5, and aluminium chloride 2.5. 6-Benzylaminopurine, alpha-naphthylacetic acid, beta-indolylbutyric acid are used by turn as growth regulating agents and at successive alternation of temperature and light regimes. Method ensures to improve development of hybrid embryos from interspecies crossings and increase the yield of bulbs by above 74-fold as compared with conditions in vivo. Invention can be used in selection and seed growing of tulips. EFFECT: improved method of preparing. 5 tbl, 1 exa