Fremgangsmåde til fremstilling af en levedygtig cellekultur, der er inficeret med en med dissemineret sklerose forbundet vi

摘要

PCT No. PCT/FR93/00337 Sec. 371 Date Feb. 2, 1994 Sec. 102(e) Date Feb. 2, 1994 PCT Filed Apr. 2, 1993 PCT Pub. No. WO93/20189 PCT Pub. Date Oct. 14, 1993The present invention relates to a process for in vitro culture of cells infected by a virus associated with multiple sclerosis and to the infected cell lines thus produced. The process is the cultivation of human cells infected by a viral strain to obtain at least one culture of primary cells infected by the said viral strain, along with the cultivation of non-infected human cells permissive to the viral strain to obtain at least one permissive culture, followed by cocultivation of at least one sample of a culture of infected primary cells and one sample of the permissive culture to obtain a first infected derived culture, then cultivating in series of the first infected derived culture. The invention is used in particular in the pharmaceutical diagnostics industry sector. In the preferred process, the infected cells are leptomeningeal cells and the permissive cells are leptomeningeal cells or plexuschoroideus cells.