摘要 |
There is disclosed a composition represented by the formula PIPn-L-S-Matrix, wherein PIPn is a phosphoinositide polyphosphate, L is a linker moiety, S is a scintillant, and Matrix is a solid support. Preferred linker moieties include succinimide and poly(ethylene glycol) linkers, and a preferred scintillant is 2-(4-amino-methylphenyl)-5-(4-biphenyl)-1,3,4-oxadiazole. In a preferred embodiment, the Matrix is a microtitre plate. The composition can be used for assaying phosphatidylinositol kinases and for screening compounds for inhibition of phosphatidylinositol kinase activity. Methods of making and using the compound are also disclosed. |