| 摘要 |
The present invention relates to a recombinant host cell comprising a gene coding for a peroxidase capable of having disulphide bonds, said gene is expressible in the cell, the cell is genetically modified to have a less reducing intracellular environment as compared to a non-modified cell. In a preferred embodiment, the cell has been modified to have a reduced or lacking activity of a thioredoxin reductase or an enzyme having a similar effect on the sulfhydryl reducing potential of the cytoplasm. Preferably, the gene coding for peroxidase is derived from a plant selected from the group consisting of a barley species, a soy bean species, a cruciferous species, such as horse radish and Arabidopsis thaliana, a Brassica species, rape and a Solanaceae species. Further, the invention relates to a process of producing an enzymatically active peroxidase capable of having disulphide bonds, the process comprising the steps of providing a cell as defined above, cultivating the cell under conditions where the gene is expressed, isolating the peroxidase, and optionally subjecting the isolated peroxidase to a folding treatment, e.g. under non-reducing conditions without altering the redox state, to obtain a proportion of enzymatically active peroxidase which, relative to the proportion of enzymatically active peroxidase which is obtained under identical conditions with the non-modified cell, is increased by at least 10 %. |
