| 摘要 |
<p>A method for conducting a receptor-ligand binding reaction of a solution containing or suspected of containing the target analyte. The method comprises the steps of bonding the first binding partner to the surface of a paramagnetic particle, conjugating a second binding partner to a calcium-sensitive luminescent compound; contacting the first and second binding partners with the solution to be tested, immobilizing the paramagnetic particles along a capture strip that has a transverse stripe containing streptavidin and containing a caged calcium compound, exposing the transverse stripe to a pulse of ultraviolet light to effect the release of calcium from the caged calcium compound, and measuring luminescence emitted by the calcium-sensitive luminescent material. The method may be used in the testing of blood. An apparatus is also disclosed.</p> |
