New synthetic derivatives of the C-peptide of proinsulin, useful in the preparation of human insulin or insulin analogs in high yield

摘要

Synthetic derivatives (I) of the C-peptide of proinsulin are new. Also new is DNA encoding (I). New precursors for human insulin or insulin analogs are of formula Fus-B(1-30)-RDVP-Yn-A(1-21) (I). Fus = optionally present fusion component of arbitrary sequence; B(1-30) = the B-chain of human insulin; Y = amino acid chain having a basic amino acid at the C-terminal; n = 2-50 (defining the chain length of Y), and A(1-21) = the A-chain of human insulin; the A- and/or B-chain(s) may be modified by amino acid exchanges, additions and/or deletions. Independent claims are included for the following: (1) DNA (II) encoding (I); (2) vectors (III) containing (II) specifically where the vector is an expression vector suitable for expression in Escherichia coli; Escherichia coli cells containing (III); and (3) preparation of (I) and (II).