METHODS OF MANIPULATING AND SEQUENCING NUCLEIC ACID MOLECULES USING TRANSPOSITION AND RECOMBINATION

摘要

The present invention relates generally to methods, kits and compositions for use in manipulating nucleic acid molecules, particularly cloning, sequencing, amplifying and mutating such molecules. In particular, the invention relates to use of recombination sites and recombinational cloning to manipulate, select and analyze nucleic acid molecules of interest.