HEPATITIS B VIRUS STRAIN, INDUCED BY VACCINE, NUCLEIC ACID ISOLATED MOLECULE CODING MUTANT MAIN SURFACE ANTIIGEN OF HEPATITIS B VIRUS, VECTOR AND VECTOR SYSTEM FOR THE PREPARATION OF POLYPEPTIDE, A METHOD FOR THE PREPARATION OF POLYPEPTIDE (VARIANTS), PURIFIED POLYPEPTIDE (VARIANTS), OLIGONUCLEOTIDE, A METHOD FOR THE PREPARATION OF ANTIBODIES (VARIANTS), ANTIBODY (VARIANTS), A METHOD FOR THE DETERMINATION OF CHEMICAL COMPOUND FOR TREATING THE INFECTION OF HEPATITIS B STRAIN INFECTION, A COMPOSITION, A METHOD FOR THE PREPARATION OF COMPOSITION, A METHOD OF ORGANISM LIQUIDS SCREENING FOR HEPATITIS B VIRUS, A METHOD OF ANTIBODY USE, VACCINE AGAINST HEPATITIS

摘要

This invention provides an isolated strain of Hepatitis B virus designated Human Hepatitis B Virus Surface Antigen-'S-145 Singapore Strain (Glycine to Arginine) which constituent viral genome is deposited under Accession Nos. P97121504, P97121505 andP97121506 with the European Collection of Cell Culture on 15th December 1997. This invention also provides an isolated nucleic acid encoding a polypeptide which is a mutant major surface antigen of a strain of hepatitis B virus, such polypeptide having an amino acid sequence which differs from the amino acid sequence of a major surface antigen of a wild type hepatitis B virus in that the amino acid at position number 145 of such polypeptide is an arginine rather than a glycine, and the punned peptide. This invention further provides an isolated nucleic acid which encodes a peptide, wherein the peptide is encoded by a nucleic acid molecule comprising nucleotides 527 through 595 of SEQ. ID. No. 1, and the purified peptide. This invention also provides various methods using the disclosed isolated nucleic acids and polypeptides. This invention also provides various uses of the viral strain and its proteins.