METHOD OF AGROBACTERIUM MEDIATED PLANT TRANSFORMATION THROUGH TREATMENT OF GERMINATING SEEDS

摘要

The invention relates to a plant transformation method through treating germinating plant seeds with an Agrobaterium strain carrying a foreign DNA fragment. In particular, germinating plant seeds were used as receptors, Ti (tumor inducing) plasmid harbored in an Agrobacterium tumefaciens strain carrying a n inserted foreign DNA fragment was used as a gene donor. The germinating plan t seeds were co-cultured with the Agrobacterium strain, during the process the foreign DNA fragment was transferred into the donor plant genome. PCR amplification and PCR-Southern hybridization verified that the foreign gene, fragment has be en transferred into the donor plant and can inherit to the next generation.