Method of identifying cellular regulators of adeno-associated virus (AAV)

摘要

The present invention relates to a genetic screening assay to identify molecules that interact with a viral regulatory element. The viral regulatory element may be derived from an adeno associated virus (AAV), and may optionally contain at least one inverted terminal repeats (ITR) or one or more regions thereof. The construct containing the viral regulatory sequence is linked to a reporter gene so that the reporter gene will be expressed in the presence of proteins or other molecules that bind to the viral regulatory element. The assay is beneficial for analyzing molecules that bind to viral regulatory regions, and may be also useful as an assay kit to examine such interactions.