发明名称 |
KINASE ACTIVITY DETECTION METHODS |
摘要 |
The invention provides a method for detecting the activities of two or more kinases. The method enables multiplexed detection with high signal to noise in a high-throughput-compatible format and a platform that could be applied to other lanthanide metal and fluorophore combinations to achieve even greater multiplexing without the need for phosphospecific antibodies. |
申请公布号 |
US2016376632(A1) |
申请公布日期 |
2016.12.29 |
申请号 |
US201615195712 |
申请日期 |
2016.06.28 |
申请人 |
REGENTS OF THE UNIVERSITY OF MINNESOTA ;PURDUE RESEARCH FOUNDATION |
发明人 |
Parker Laurie L.;Lipchik Andrew M. |
分类号 |
C12Q1/48 |
主分类号 |
C12Q1/48 |
代理机构 |
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代理人 |
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主权项 |
1. A method for detecting the activities of two or more kinases comprising:
a) contacting a first kinase and a second kinase with a first peptide and a second peptide, wherein:
i) the first peptide is a substrate for the first kinase;ii) the second peptide is a substrate for the second kinase;iii) each peptide is associated with a lanthanide;iv) each peptide comprises a group capable of sensitizing the lanthanide that is associated with that peptide; andv) each peptide is linked to a fluorophore under conditions such that a first signal associated with the activity of the first kinase and a second signal that is associated with the activity of the second kinase are generated; and b) detecting the first signal and the second signal. |
地址 |
Minneapolis MN US |