发明名称 |
Methods and compositions for detecting cancers associated with methylation of hMLH1 promoter DNA |
摘要 |
Methods are provided for detection of cancers associated with methylation of hMLH1 promoter DNA in a subject. The method comprise assaying for the presence of methylated hMLH1 promoter DNA in a bodily fluid from a subject. In one embodiment, the method comprises reacting DNA from the sample with a chemical compound that converts non-methylated cytosine bases but not methylated cytosine bases, to a different nucleotide base. The compound-converted DNA is then amplified using a methylation-sensitive polymerase chain reaction (MSP) employing primers that amplify the compound-converted DNA template. The present invention also provides nucleotide primer sequences for use in the methylation-sensitive PCR assay.
|
申请公布号 |
US2003068620(A1) |
申请公布日期 |
2003.04.10 |
申请号 |
US20010957661 |
申请日期 |
2001.09.20 |
申请人 |
MARKOWITZ SANFORD;GRADY WILLIAM |
发明人 |
MARKOWITZ SANFORD;GRADY WILLIAM |
分类号 |
A61B;C07H21/02;C07H21/04;C12P19/34;C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
A61B |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|