HYBRIDIZATION METHOD AND APPARATUS

摘要

PROBLEM TO BE SOLVED: To provide a hybridization method and apparatus to improve the hybridization efficiency of a target DNA relative to the probe DNA immobilized on a substrate surface in a DNA chip assay. SOLUTION: A hybridization solution containing fine particles is sealed on a DNA chip with a cover glass and a sealing agent. The hybridization chamber holding the DNA chip is rotated on a rotary shaft. Since the fine particles are moved in the direction of the gravitation force, these particles have an effect to stir the hybridization solution. The hybridization efficiency is increased by the increase of the diffusion distance of the DNA target in the hybridization solution. The method is effective for improving the hybridization efficiency in the DNA chip assay and attains a signal having high quantitative accuracy and reproducibility. COPYRIGHT: (C)2004,JPO