发明名称 |
Rekombinant DNA-molekyle, transformant, 2,5-DKG-reduktase, fremgangsmåde til fremstilling af en 2,5-DKG-reduktase, fremgangsmåde til fremstilling af 2-KLG ud fra glucose samt fremgangsmåde til fremstilling af vitamin C |
摘要 |
An enzyme for conversion of 2,5-diketo-D-gluconate (2,5-DKG) to 2-keto-L-gulonic acid (2-KLG) and a genetically modified organism that expresses all the fermentation enzymes needed to convert glucose to 2-KLG (a precursor to ascorbic acid) using the new enzyme. Preferably, the organism is Erwinia citreus, or a mutated strain of Erwinia citreus, unable to use 2,5-DKG or 2-KLG as a sole carbon source, into which the gene for a 2,5-DKG reductase, produced by Corynebacterium sp., SHS 752001, has been inserted. The preferred transformed organism expresses the fermentation enzymes Erwinia citreus normally expresses for fermentation of glucose to 2,5-DKG and, in addition, an enzyme Corynebacterium sp. SHS 752001 expresses for fermentation of 2,5-DKG to 2-KLG. |
申请公布号 |
DK175120(B1) |
申请公布日期 |
2004.06.07 |
申请号 |
DK19870001694 |
申请日期 |
1987.04.02 |
申请人 |
GENENCOR INTERNATIONAL, INC. |
发明人 |
HARDY, KIMBER;POL, HENDRICK VAN DE;GRINDLEY, JUNE;PAYTON, MARK |
分类号 |
C12N1/21;C12N1/20;C12N9/04;C12N15/00;C12N15/09;C12N15/53;C12P7/58;C12P7/60;C12P17/04;C12P19/02;C12R1/15;C12R1/18;C12R1/19;(IPC1-7):C12N15/53 |
主分类号 |
C12N1/21 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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