РЕКОМБИНАНТНАЯ ПЛАЗМИДНАЯ ДНК PET23-A(+)PRXVIHUMΔ178, КОДИРУЮЩАЯ N-КОНЦЕВОЙ ФРАГМЕНТ ПЕРОКСИРЕДОКСИНА VI ЧЕЛОВЕКА И ШТАММ E. COLI BL21/DE3/PET23-A(+)/PRXVIHUMΔ178-ПРОДУЦЕНТ N-КОНЦЕВОГО ФРАГМЕНТА ПЕРОКСИРЕДОКСИНА VI ЧЕЛОВЕКА

摘要

FIELD: biotechnology, genetic engineering, pharmaceutical industry. ^ SUBSTANCE: plasmid DNA pET23-a(+)/PrxVIhumDelta178 with molecular weight of 19691.61 Da is constructed. DNA contains RNA-polymerase T7 promoter; replication initiation site; genetic marker which determinates resistance of cells transformed by said plasmid to ampicillin; and nucleotide sequence encoding N-terminal fragment of human peroxiredoxine VI containing 177 of amino acid residues. E.coli strain BL21/DE3/pET23-a(+)/PrxVIhumDelta178 being producer of N-terminal fragment of human peroxiredoxine VI is obtained by transformation of E.coli cells with plasmid DNA pET23-a(+)/PrxVIhumDelta178. Method of present invention makes it possible to obtain human peroxiredoxine VI fragment having reduced molecular weight, improved tissue permeability, and antioxidant activity of full-scale peroxiredoxine. ^ EFFECT: human peroxiredoxine VI fragment with improved tissue permeability. ^ 2 cl, 3 dwg, 4 ex