| 摘要 |
The invention relates to a method of detecting a target nucleic acid by linear amplification. The invention also relates to a method of detecting a target nucleic acid by amplification. The method of the invention includes the steps of forming at least a first cleavage structure comprising at least one flap, cleaving the cleavage structure with a cleavage means to release the at least one flap and detecting the at least one flap. In one embodiment of the invention, the method includes the additional steps of forming at least a second cleavage structure comprising the at least one released flap from the first cleavage structure cleaving the second cleavage structure with a cleavage means to release at least one flap from the second cleavage structure and detecting released flaps from the first or second cleavage structure.
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