| 摘要 |
The method of manufacture of a tissue matrix is described, which is to be used for implantation into a patient. This comprises collecting embryonic stem cells from a placenta which has been treated to remove residual cord blood and seeding them onto or into a tissue matrix for implantation into a patient. Also disclosed is perfusing a placenta with anticoagulant solution to flush out residual cells and collecting them and perfusion fluid from the drained placenta for seeding into or onto the tissue matrix. Use of a seeded tissue matrix is for the manufacture of an implant for treating a patient to repair or replace tissue wherein the implant is adapted to be implanted at a site of the patient's body in need of treatment. A pharmaceutical composition containing an isolated placental stem cell, obtained by exsanguinating, perfusing and then incubating the placenta, is also disclosed. |
