Recombinational cloning using nucleic acids having recombination sites

摘要

Disclosed is a composition comprising at least one topoisomerase bound to a nucleic acid encoding a recombination site. Also disclosed is a nucleic acid comprising one or more site specific recombination sites and a topoisomerase recognition sites. The recombination site is preferably an att site or a lox site, and if there are multiple recombination sites, they preferably do not recombine with each other. (62) Divided out of 520579