Method and apparatus for measuring liquid level of cell culture solution

摘要

A method of measuring a liquid level of a cell culture solution stored in a cell vessel, includes: irradiating the cell culture solution with at least two kinds of light beams including a first light beam having a first wavelength and a second light beam having a second wavelength; measuring a first absorbance of the cell culture solution with respect to the first light beam, and a second absorbance of the cell culture solution with respect to the second light beam; and determining a liquid level of the cell culture solution based on the first absorbance and the second absorbance.

主权项

1. A method of measuring a liquid level of a cell culture solution stored in a cell vessel, the method comprising:
irradiating the cell culture solution with at least two kinds of light beams including a first light beam having a first wavelength and a second light beam having a second wavelength; measuring a first absorbance of the cell culture solution with respect to the first light beam, and a second absorbance of the cell culture solution with respect to the second light beam; and determining a liquid level of the cell culture solution based on the first absorbance and the second absorbance, wherein the at least two kinds of light beams are selected from four kinds of light beams having different wavelengths, in accordance with a kind of the cell culture solution, and wherein the cell culture solution contains:
a first material having a first absorption peak within a wavelength region of 300 to 800 nm; anda second material having a second absorption peak or a first convergence point where an absorbance converges to a constant value irrespective of a pH, and a third absorption peak or a second convergence point where an absorbance converges to a constant value irrespective of a pH, within the wavelength region, wherein the four kinds of light beams include:
a light beam having a wavelength corresponding to the first absorption peak;a light beam having a wavelength corresponding to the second absorption peak or the first convergence point;a light beam having a wavelength corresponding to the third absorption peak or the second convergence point; anda light beam having a wavelength at which an absorbance of at least one of the first material and the second material converges to a constant value irrespective of a pH, and wherein a pH of the cell culture solution is determined by measuring absorbances of the cell culture solution with respect to the four kinds of light beams.