| 摘要 |
The present invention relates to a process for the enzymatic modification of a glycoprotein. The process comprises the step of contacting a glycoprotein comprising a glycan comprising a terminal GlcNAc-moiety, in the presence of glycosyltransferasethat is,or is derived from,a β-(1,4)-N-acetylgalactosaminyltransferase, with anon-natural sugar-derivative nucleotide. Thenon-natural sugar-derivative nucleotide is according to formula (3): wherein A is selected from the group consisting of -N3, -C(O)R3, -(CH2)iC≡C-R4, - SH, -SC(O)R8, -SC(0)OR8, -SC(S)OR8, -F, -CI, -Br -I, -OS(O)2R5, terminal C2 - C24 alkenyl groups, C3 - C5 cycloalkenyl groups, C4 - C8 alkadienyl groups, terminal C3 - C24 allenyl groups and amino groups. The invention further relates to a glycoprotein obtainable by the process according to the invention, to a bioconjugate that can be obtained by conjugating the glycoprotein with a linker-conjugate, and to β-(1,4)-Ν- acetylgalactosaminyltransferases that can be used in preparing the the glycoprotein according to the invention. |
