Methods for producing proteins in plants

摘要

The present invention relates to methods for expressing proteins of interest, particularly pharmaceutically valuable proteins, transiently in plants. In particular, the invention provides an improved method for introducing Agrobacterium cells into a whole plant or a plant organ. The methods of the invention provides efficient agroinfiltration of many plants singly or simultaneously resulting in a yield of recombinant proteins that is higher than that obtained by other methods. The methods can be readily scaled and automated to meet changing demands of the recombinant protein.

主权项

1. A system for infiltrating Agrobacterium bacteria into a whole and intact plant or a part of a whole and intact plant and/or for producing a heterologous peptide or protein in a whole and intact plant or a part of a whole and intact plant comprising:
a) a chamber defining an interior and comprising
(i) an inlet in communication with the interior of the chamber;(ii) an opening lined with an elastic seal;(iii) an outlet in communication with the interior of the chamber; and b) a source of compressed air operably coupled to the inlet, wherein said chamber is configured for receiving through the opening all or part of areal parts of a plant and wherein the elastic seal is configured such that a seal can be formed around the main stem of the plant such that all or part of the aerial parts of a plant is positioned inside the chamber.