Method for preparing biological tissues for use in biological prostheses

摘要

A method of treating a biological tissue for biological prostheses includes steps of fixation of the biological tissue via a fixing solution including glutaraldehyde and detoxification of the fixed biological tissue via treatment with a detoxifying solution. The detoxification step includes one or both of eliminating phospholipids via treatment with an elimination solution and a treatment with a detoxifying solution. The elimination solution includes 1,2-octanediol and ethanol. The detoxifying solution includes taurine or homocysteic acid.

主权项

1. A method of treating a biological tissue for use in a biological prosthesis, the method comprising the steps:
eliminating phospholipids from the biological tissue by treating the biological tissue with an elimination solution at a temperature of 15° C. to 65° C., the elimination solution including 1,2-octanediol at a concentration of 1% v/v to 25% v/v and ethanol at a concentration of 25% to 49% v/v in a buffer at a pH of 4 to 9; fixing the biological tissue treated with the elimination solution by treating the biological tissue, the fixing solution including glutaraldehyde at a concentration of 0.10% v/v to 2.00% v/v in a buffer at a pH of 5 to 8; and detoxifying the biological tissue treated with the fixing solution by treating the biological tissue with a detoxifying solution at a temperature above 20° C., the detoxifying solution including taurine or homocysteic acid in a buffer at a pH of 4 to 9.