A BIGENIC MAMMALIAN EXPRESSION VECTOR

摘要

The present invention provides a bi-cistronic bigenic mammalian expression vector. This mammalian expression vector is comprised of three transcription cassettes, transcription cassette one for expression of first polypeptide of interest, transcription cassette two for expression of DHFR gene and transcription cassette three for expression of second polypeptide of interest. The present vector also have atleast two different antibiotic selection marker genes and atleast one matrix attachment region (MAR). The expression vector facilitates high level expression of polypeptides of interest and easy and cost effective selection of high producing host cell clones.