METHODS AND SYSTEMS FOR BIOLOGICAL INSTRUMENT CALIBRATION

摘要

In one exemplary embodiment, a method (100) for calibrating an instrument is provided. The instrument includes an optical system capable of imaging florescence emission from a plurality of reaction sites. The method includes performing a region-of-interest (ROI) calibration (102) to determine reaction site positions in an image. The method further includes performing a pure dye calibration (108) to determine the contribution of a fluorescent dye used in each reaction site by comparing a raw spectrum of the fluorescent dye to a pure spectrum calibration data of the fluorescent dye. The method further includes performing an instrument normalization calibration (110) to determine a filter normalization factor. The method includes performing an RNase P validation (112) to validate the instrument is capable of distinguishing between two different quantities of sample.