| 摘要 |
PURPOSE:To enable stable detection of trace components for a short space of time by precise separation with a capillary tube small in the pipeline diameter after course separation with a pretube large therein. CONSTITUTION:A pipeline for electrophoresis between a sample injection section 6 and a detector 7 is composed of a 2-stage tube 11 in which a pretube 8 large in the pipeline diameter is connected in series to a capillary tube 9 small therein. An interface between a terminal liquid and a leading liquid is formed at the sample injection section 6 and a given amount of a sample is injected thereinto. A microcomputer 14 controls a constant current power supply circuit 2 and a switching circuit 16 to supply a constant current between a terminal liquid electrode cell 3 and a second leading liquid electrode cell 5 for a given time. After a part slightly before the rear end of an ion component zone of the sample reaches a connecting step 10, the microcomputer 14 controls the constant current power supply circuit 2 and the switching circuit 16 to supply a constant current between the terminal liquid electrode cell 3 and the first leading liquid electrode cell 4 so that it will be precisely separated with the capillary tube 9. |
