NOVEL GLUCOSE OXIDASES DERIVED FROM ASPERGILLUS NIGER

摘要

Novel glucose oxidase variants are disclosed that have besides the two substitutions T30V and I94V according to SEQ ID NO:1, additionally at least one further amino acid substitution in the polypeptide sequence in any of the positions S53; A137; A173; A332; F414 and V560. The glucose oxidase variants herein exhibit specificity for glucose and significantly reduced oxygen consumption rates and/or increased enzyme activity for electron mediators other than oxygen. Also provided are assay devices incorporating at least one of the GOx variants herein for improved blood glucose measurements.