DETACHMENT OF ANCHORAGE-DEPENDENT CELLS FROM MICROCARRIERS
摘要
A method for detaching cells from positively-charged cell culture microcarriers. In this method microcarriers laden with attached cells are exposed to a proteolytic agent at relatively high pH and then subjected to mild shear.
申请公布号
WO8601531(A1)
申请公布日期
1986.03.13
申请号
WO1985US01615
申请日期
1985.08.27
申请人
MASSACHUSETTS INSTITUTE OF TECHNOLOGY
发明人
GIARD, DONALD, J.;HU, WEI-SHOU;WANG, DANIEL, I., C.