| 摘要 |
A method for electrically immortalizing lymphoid cell lines through the introduction of cloned cellular oncogenes by high voltage electric pulses. A human B-lymphocyte suspension is placed between two electrodes. Naked DNA containing a cellular oncogene, preferably human c-myc, is added to the cell suspension. An electric potential is applied to the electrodes in a pulse of from about 0.1 nanosecond to 10 milliseconds to create an electric field of about 1 to about 40 kV/cm, thereby introducing the oncogene into at least some of the cells to immortalize those cells. Also disclosed are immortalized B-lymphoid cells containing an exogenous, immortalizing cellular oncogene. |
