Illumination apparatus optimized for synthetic aperture optics imaging using minimum selective excitation patterns

摘要

A synthetic aperture optics (SAO) imaging method minimizes the number of selective excitation patterns used to illuminate the imaging target, based on the objects' physical characteristics corresponding to spatial frequency content from the illuminated target and/or one or more parameters of the optical imaging system used for SAO. With the minimized number of selective excitation patterns, the time required to perform SAO is reduced dramatically, thereby allowing SAO to be used with DNA sequencing applications that require massive parallelization for cost reduction and high throughput. In addition, an SAO apparatus optimized to perform the SAO method is provided. The SAO apparatus includes a plurality of interference pattern generation modules that can be arranged in a half-ring shape.

主权项

1. An apparatus for performing synthetic aperture optics (SAO) on a target including one or more objects, the apparatus comprising:
a plurality of interference pattern generation modules (IPGMs), each IPGM configured to generate a pair of light beams that interfere to generate a selective excitation pattern on the target at a predetermined orientation and a predetermined pitch; a monolithic half-ring shaped structure holding the plurality of IPGMs; and an optical imaging module configured to optically image the illuminated target at a resolution insufficient to resolve the objects, the optical image of the illuminated target being further processed using information on the selective excitation pattern to obtain a final image of the illuminated target at a resolution sufficient to resolve the objects.