| 摘要 |
PURPOSE:A strain in Serratia, requiring succinic acid or resisting nucleic acid base analogs, and producing the titled substance, is cultured and the titled substance is collected from the culture mixture. CONSTITUTION:A mutant in Serratia which requires succinic acid or resists nucleic acid base analogs such as Serratia marcescens SA268 or Cerratia marcescens AUr-116 is cultured in a medium for L-arginine production. The culture medium contains glucose, fumaric acid, alcohol, ammonium sulfate, urea, pepton, fish meat extract, inorganic salts and, when a succininc acid-requiring mutant is employed, further sodium succinate, ammonium succinate or other analogous salt is added. The cultivation is carried out aerobically at 20-40 deg.C and 6-8pH and, after completion of cultivation, insoluble substances are removed, then separation and purification are conducted by means of ion-exchange resins to give L-arginine. |
