发明名称 |
HYBRIDISATION METHOD AND PROBES THEREFOR |
摘要 |
A method for discriminating between two complementary nucleotide sequences differing by as little as 1-3 nucleotides is described in which an oligonucleotide signalling complex probe is used, which probe comprises a single stranded oligonucleotide of 8 to 30 nucleotide units having covalently attached thereto, at a sterically tolerant site, a residue capable of producing a non-radioisotopic signal and having a molecular weight of at least 100 daltons. Oligonucleotide signalling complex probes and diagnostic kits for use in such a method are described and claimed. |
申请公布号 |
ZA8602583(B) |
申请公布日期 |
1987.02.25 |
申请号 |
ZA19860002583 |
申请日期 |
1986.04.07 |
申请人 |
IMPERIAL CHEMICAL INDUSTRIES PLC. |
发明人 |
FRANCIS JOSEPH CARR;MICHAEL DEREK EDGE |
分类号 |
G01N33/50;C07H21/00;C12N15/09;C12Q1/68 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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