| 摘要 |
<p>A fermentation process is disclosed for the preparation of an inoculum, suitable for batchwise, semicontinuous, or continuous production of coenzyme B12 under septic conditions. The fermentation process begins with a broth consisting of methanol, cornsteep liquor hydrolysate or heat-treated corn slop, ammonium bicarbonate, magnesium chloride, cobalt chloride, 5,6-dimethyl-benzimidazole and sodium bisulfite used for anaerobic, mesophilic, septic fermentation production of coenzyme B12 with anaerobic, digested sewage sludge. Fermentation is carried out over several steps to provide an inoculum containing an anaerobic, mesophilic, methane-producing, new mixed micropopulation, containing bacteria of each of the types deposited at the Hungarian National Collection of Medical Bacteria, National Institute of Hygiene, under numbers 00076, 00079, and 00272.</p> |
