| 摘要 |
PROBLEM TO BE SOLVED: To provide methods of purifying Fc-binding proteins (particularly Fc-binding proteins derived from human FcγRIIIa) with high purity and high efficiency using cation exchange chromatography.SOLUTION: The invention relates to a production method of Fc-binding proteins, wherein the cation exchange chromatography carrier, to which Fc-binding protein is adsorbed, is washed with a first washing solution containing less than 150 mmol/L of sodium chloride and subsequently washed with a second washing solution containing sodium chloride with a concentration that is higher than that of the first washing solution and is 150 mmol/L or less; and the Fc-binding protein is eluted from the carrier with an eluate containing 250 mmol/L or more of sodium chloride.SELECTED DRAWING: None |
