发明名称 |
Polycationic buffers and method for gel electrophoresis of nucleic acids. |
摘要 |
<p>The present invention provides for polycationic buffers for the gel electrophoresis of and methods for obtaining improved separation and resolution of nucleic acids. In particular, the invention provides for a buffer consisting essentially of histidine for the gel electrophoresis of oligonucleotides ranging from a few to about 70 nucleotides in length. Polycationic buffers may be used for both analytical and preparative purposes and result in a nearly two-fold improvement in both separation and resolution of the nucleic acid bands. Furthermore, the use of polycationic buffers reduces the electrophoresis time by a factor of about three due to the low conductivity of the buffers.</p> |
申请公布号 |
EP0292837(A1) |
申请公布日期 |
1988.11.30 |
申请号 |
EP19880107864 |
申请日期 |
1988.05.17 |
申请人 |
ABBOTT LABORATORIES |
发明人 |
MANDECKI, WLODEK;HAYDEN, MARK A. |
分类号 |
C07H21/04;G01N27/26;G01N27/447;(IPC1-7):C12Q1/68 |
主分类号 |
C07H21/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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